Stoichiometry of microorganisms
Although cell growth is a complex process, it must follow the rules of mass and energy conservation. Nutrients and organic compounds and other life elements are involved with the metabolism activities. As a result, the cells grow, the energy is produced, the biological synthesis is done and the products are formed. From the examples above, the law of mass survival can be used successfully to measure unknown components. Cell growth also follows the crime survival law. Essences in life essentially consist of four main elements: O, H, C, and N. These are the four main elements in the processes of cell metabolism. Bio-growth products are aerobic, carbon dioxide and H 2 O. Each element is balanced by the amount exported. In other words, the amount of mercury produced or the amount of heat released by cell growth, with the amount of substrate consumed and CO 2Produced, fit. Since the metabolites and organic compounds are different from the cellular material released into the environment, the environment of the nondestine and substrate is omitted.
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As a result, cell growth and inflammation are achieved. Considering the cell from a macroscopic point of view, consider the cell as a system in which cell growth is independent of the pathways of biochemical reactions. By applying the overall growth response, the substrate penetrates into the system due to the difference in concentration with the substrate and the extra biomass is removed from the original system and cellular growth is achieved.
Cell growth and metabolism activities are described in a similar way to a simple reaction. It is also necessary to make a definite formula for dry cells. The elemental composition of a specific species of living microorganism is defined by the experimental formula CH α O β N δ . The overall biochemical reaction to biomass production is based on the use of a bio-substrate, as shown below. The oxidation of the substrate in the following biochemical oxidation is simplified:
Approximately 56% of Escherichia coli, O, H, C, and N are. The chemical formula of cell composition and stoichiometric coefficients in the high reaction depend on the composition of the culture medium and the surrounding environment. All the main elements in the equation above must be balanced. Then the stoichiometric coefficients are determined by solving a system of equations simultaneously.
Note that there are five unknowns (a, b, c, d, e), but we have four equations. This means that in order to solve the system of five equations with four unknowns, we need an additional equation. An important and measurable parameter in a living system is the respiratory efficiency (RQ), which is absorbed as the CO 2 molecules produced in the molar O 2 .
Combining the chemical percentage of the species of live microorganisms, the composition of the percentage of the components of the environment depends. Even for a single living creature such as Escherichia coli, which has been grown in different environments, if the composition of the percentages of the components of the environment is different, the percentage composition of the O, N, C, and H is different. There are a few environmental factors that are known to limit the growth rate and affect cell growth and environmental conditions. If a specific neutrin in the environment suddenly increases, does the growth rate of the cell increase? At first, it is essential to have a brief discussion of the resources needed to grow germs, then respond directly to the hints. Occasionally, the percentage composition of the environment is based on a single component that has a limited growth track.
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This means that a change in the specific concentration of specific norepinol may affect cell growth, and the depletion of specific nourinum can delay the growth pattern. Growth can also be controlled by the produced product. On the other hand, changes in the concentration of environmental compositions cause relatively modest changes in the growth trajectory. In fact, this can not be right, because cell growth does not depend on the single substrate. Even cell growth is developed on yeast extracts. Cellular metabolism is not limited to carbohydrate catabolism. Lipids, lipids, and proteins are involved with energy production for biological synthesis. The elemental composition of the selected microorganisms can be defined as follows When RQ is given, stoichiometric coefficients can be calculated simultaneously
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