Principles and Techniques of Biochemistry Seventh edition Pdf Free Download
EDITED BY KEITH WILSON AND JOHN WALKER
Principles and Techniques of Biochemistry and Molecular Biology Seventh edition Pdf Free Download This new edition of the bestselling textbook integrates the theoretical principles and experimental techniques common to all undergraduate courses in the bio- and medical sciences. Three of the 16 chapters have new authors and have been totally rewritten. The others have been updated and extended to reﬂect developments in their ﬁeld exempliﬁed by a new section on stem cells.
Two new chapters have been added. One of the clinical biochemistry discusses the principles underlying the diagnosis and management of common biochemical disorders. The second one on drug discovery and development illustrates how the principles and techniques covered in the book are fundamental to the design and development of new drugs. In-text worked examples are again used to enhance student
understanding of each topic and case studies are selectively used to illustrate important examples. Experimental design, quality assurance and the statistical analysis of quantitative data are emphasized throughout the book.
1 Basic principles 1 K. WILSON 1.
1 Biochemical and molecular biology studies 1 1.
2 Units of measurement 3 1.
3 Weak electrolytes 6 1.
4 Quantitative biochemical measurements 16 1.
5 Safety in the laboratory 35 1.
6 Suggestions for further reading 372
Cell culture techniques
38 A.R. BAYDOUN
2.1 Introduction 38 2.2 The cell culture laboratory and equipment 39 2.3 Safety considerations in cell culture 43 2.4 Aseptic techniques and good cell culture practice 44 2.5 Types of animal cell, characteristics and maintenance in culture 49 2.6 Stem cell culture 61 2.7 Bacterial cell culture 68 2.8 Potential use of cell cultures 71 2.9 Suggestions for further reading 72
3 Centrifugation 73
K. OHLENDIECK 3.
1 Introduction 73 3.
2 Basic principles of sedimentation 74 3.
3 Types, care and safety aspects of centrifuges 79 3.
4 Preparative centrifugation 86 3.
5 Analytical centrifugation 95 3.
6 Suggestions for further reading4 Microscopy 100
S. W. PADDOCK 4.
1 Introduction 100 4.
2 The light microscope 103 4.
3 Optical sectioning 116 4.
4 Imaging living cells and tissues 123 4.
5 Measuring cellular dynamics 126 4.
6 The electron microscope (EM) 129 4.
7 Image archiving 133 4.
8 Suggestions for further reading 1365 Molecular biology,
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