Microbial cell stabilization method

Microbial cell stabilization method




Microorganisms are biocatalysts used in the fermentation process, which ferment fermentable carbohydrates, produce crop and biomass. The cells are separated and reused. Stabilization of cells has been used in vinegar production. Enzymes and all cells can be bonded to a solid filled substrate and stabilized like an active layer. When the substrate passes through the surface, the enzymatic reaction of the substrate turns into a desirable product. Enzymes and microorganisms are suitable for the production of good foods, additives, medicines and other substances produced by microbial metabolites. Stabilized cells may be different from cells that are freely suspended. They may produce outer cell polymers that keep the cells close to measuring the dry cell weight. The result is an overestimation of the biocompatibility. In addition, the location of the cell, in the formation of biofilms, can affect quality and activity, as the profile of environmental conditions, such as the effect of substrate, is usually present. However, in many of the results of studies for suspension cultures for the model of stabilized cell bioreactors, generally, regardless of the number of biomarkers in the system. These models are usually allowed to enter different biofilm limits and it is assumed that stabilized cells are of the same quality with cell suspension and regardless of the position in the biofilm.




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Indeed, gradients of clear cellular concentration may exist for biofilm stabilized cells. Cells are placed near food to remain at the highest quality and activity compared to cells located far away, which causes differences in the activity and quality of the established cell population. This difference becomes longer when there are masses with more nutritional needs. In practice, there may be zero concentrations within the biofilm, because in these locations cellular physiology may be completely different from free and suspended cells. The activity of cells is described by the math model based on microbial kinetics and microfilm biofilms. The use of this model determines that the biomass at the external surface of the biofilm has greater activity than the biomass near the solid retaining surface, and these conditions may occur after the biofilm reaches critical thickness or forms several layers of biofilm. Determined. However, no experimental results were obtained in the field of stabilization studies for the effect of the predicted model, nor did it provide a complete method of tedium biosynthesis with suspended cells.




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Wang [2] et al., Najafpour and colleagues established by the microbial cells Nytrvbaktr Jyys , Saccharomyces cerevisiae, and Pseudomonas Rzhynvsa the gel beads are Krzh work. They separately found that more than 90% of the post-consolidated activity was maintained using the specific oxygen-consuming power (SOUR) [per mg of oxygen per gram of dry bulk / h] as a biosecurity activity detector. . Such differences are fixed in the bulk population and the activity between free and stabilized bulk is strongly dependent on the particles and the interaction between them is dependent on the holder of the matrix

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