Culture of Animal Cells A Manual of Basic Technique and Specialized Applications, Sixth Edition

Culture of Animal Cells A Manual of Basic Technique and Specialized Applications, Sixth Edition 1

Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments in the field as well as the basic skills and protocols.

Culture of Animal Cells A Manual of Basic Technique and Specialized Applications, Sixth Edition 2

Culture of Animal Cells A Manual of Basic Technique and Specialized Applications, Sixth Edition This is the sixth edition of the leading text in the basic methodology of cell culture, worldwide. Rigorously revised, it features updates on specialized techniques in stem cell research and tissue engineering; updates on molecular hybridization, somatic cell fusion, hybridomas, and DNA transfer; new sections on vitrification and Organotypic Culture, and new chapters on epithelial, mesenchymal, neuroectodermal, and hematopoietic cells; germs

Molecular Cell Biology 8th Edition

cells/stemcells/amniocytes; and non-mammalian/avian cells. It is written for graduate students, research and clinical scientists, and technicians and laboratory managers in cell and molecular biology labs and genetics labs.

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Culture of Animal Cells A Manual of Basic Technique and Specialized Applications, Sixth Edition 3

Contents

Culture of Animal Cells A Manual of Basic Technique and Specialized Applications, Sixth Edition Introduction 1 Training Programs 11 Biology of Cultures Cells 31 Laboratory design and Layout 43 Equipment 55 Aseptic Technique 73 Safety, Bioethics, and Validation 87 Culture Vessels and Substrates 105 Defined Media and Supplements 115 Serum-Free Media 129 Preparation and Sterilization 145 Primary Culture 175 Subculture and Cell Lines 199 Cloning and Selection 217 Cell Separation 237 Characterization 247 Differentiation 281

Principles and Techniques of Biochemistry Seventh edition Pdf Free Download

Transformation and Immortalization 291 Contamination 307 Cryopreservation 321 Quantitation 335 Cytotoxicity 359 Culture of Specific Cell Types 375 Culture of Tumor Cells 421 Organotypic Culture 435 Scale-Up 451 Specialized Techniques 467 Problem Solving 503 In Conclusion

Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments in the field as well as the basic skills and protocols.

An Introduction for Pharmacists and Pharmaceutical

Culture of Animal Cells A Manual of Basic Technique and Specialized Applications, Sixth Edition This eagerly awaited edition reviews the increasing diversity of the applications of cell culture and the proliferation of specialized techniques and provides an introduction to new subtopics in mini-reviews. New features also include a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling and barcoding, as well as some new specialized protocols. Because of the continuing expansion of cell culture, and to keep the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material

Culture of Animal Cells A Manual of Basic Technique and Specialized Applications, Sixth Edition

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