CONTAMINATION IN ANIMAL CELL CULTURE

CONTAMINATION IN ANIMAL CELL CULTURE

CONTAMINATION IN ANIMAL CELL CULTURE

Introduction-

we can define to contamination as the impurity in a cell culture produced by chemicals, microbes, other cells called contamination. that may be several types; like chemical contaminant witch created by some harmful chemical as sera, endotoxins, detergents, etc, biological agents like the virus, bacteria, yeast comes under microbial contamination and cross-contamination by other cell lines during culture process. that impossible to eliminate completely to them from culture but we can overcome there affect on culture by using aseptic methods,

ANIMAL CELL CULTURE MAINTENANCE

Cross-Contamination-

microbes affect cell lines quality during culture but some cell lines also have a high growing capacity like HELA or other cell lines cause to contamination. to avoid this problem before culture to cells used the aseptic technique to overcome this problem. DNA fingerprint, isotype analysis, karyotype analysis help to detect cross-contamination in cell lines,

Microbes like a virus, bacteria, yeast, mycoplasma, also responsible for biological contamination in cell culture they infected to cells and also secret toxins witch damage to cells or reduce the quality of proliferation and differentiation in culture,

Chemical Contamination-

in this type of contamination include those chemicals like detergents, endotoxins, sera etc which affect cell nature and cause impurity in culture,

Prevention of Contamination-

contamination in cell culture is a very critical condition so before use to cells detected to contamination type and its majority in cells and handle to culture process very safely to avoid infection in cells. if cells got contamination uses antibiotics to avoid or overcome this situation in culture,

ANIMAL CELL CULTURE MEDIA TYPES COMPOSITION

Used to aseptic technique and condition during culture process to avoid to contamination like use to ultraviolet rays to elimination lab air contamination, use disinfectants to clean the surface of equipment or laminar air flow or many ways to avoid to such types of contamination,

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PLANT TISSUE CULTURE TECHNIQUES