Chromosome DNA Isolation from Bacteria pdf
CTAB/NaCl solution: 10% CTAB in 0.7 M NaCl
Dissolve 4.1 g NaCl in 80 ml distilled H2O and slowly add 10g CTAB (Sigma M-7635) while heating and stirring. Adjust final volume to 100 ml.
CTAB: Hexadecyl trimethyl ammonium bromide; Cetyl trimethyl ammonium bromide; Cetrimonium Bromide; Cetab; Centimide
Proteinase K (10 mg/ml)
|1 M Tris-HCl (pH8.0)||0.1 ml|
|CaCl2·2H2O||0.0384 g||if CaCl2, 0.029 g|
|H2O||to 10 ml|
Dissolve 100 mg Proteinase K in 10 ml of Buffer. Aliquot and store at -20oC.
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