Chromosome DNA Isolation from Bacteria pdf

CTAB/NaCl solution: 10% CTAB in 0.7 M NaCl

Dissolve 4.1 g NaCl in 80 ml distilled H2O and slowly add 10g CTAB (Sigma M-7635) while heating and stirring. Adjust final volume to 100 ml.

CTAB: Hexadecyl trimethyl ammonium bromide; Cetyl trimethyl ammonium bromide; Cetrimonium Bromide; Cetab; Centimide

Proteinase K (10 mg/ml)

Buffer

Glycerol5 ml
1 M Tris-HCl (pH8.0)0.1 ml
CaCl2·2H2O0.0384 gif CaCl2, 0.029 g
H2Oto 10 ml

Dissolve 100 mg Proteinase K in 10 ml of Buffer. Aliquot and store at -20oC.

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