Chromosome DNA Isolation from Bacteria pdf
CTAB/NaCl solution: 10% CTAB in 0.7 M NaCl
Dissolve 4.1 g NaCl in 80 ml distilled H2O and slowly add 10g CTAB (Sigma M-7635) while heating and stirring. Adjust final volume to 100 ml.
CTAB: Hexadecyl trimethyl ammonium bromide; Cetyl trimethyl ammonium bromide; Cetrimonium Bromide; Cetab; Centimide
Proteinase K (10 mg/ml)
Buffer
| Glycerol | 5 ml | |
| 1 M Tris-HCl (pH8.0) | 0.1 ml | |
| CaCl2·2H2O | 0.0384 g | if CaCl2, 0.029 g |
| H2O | to 10 ml |
Dissolve 100 mg Proteinase K in 10 ml of Buffer. Aliquot and store at -20oC.
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