Chromatography Introduction Pdf Bsc Biotechnology 1st semester
Chromatography Introduction- Usually, a biological sample is made up of more than one component (inorganic samples as well). Some studies require the separation of these components from the mixture. Chromatography is a technique that can separate the different components in the sample.
Chromatography makes use of a mobile phase and a stationary phase. The stationary phase remains static or fixed (column, paper, etc) as the mobile phase moves over the stationary phase. The sample or the mixture is dissolved in the mobile phase.
The components of the sample carried by the mobile phase, get separated based on their difference in the affinity for the mobile phase and the solid phase. The sample or the mixture that has been separated into individual components is called an analyte.