Cell destruction

Cell destruction

The downstream process of fermentation is usually started by separating the cells by centrifuging the filter. In the centrifugal operation, a vertical wheel, a horizontal wheel or even a plate type are used. Sedimentation or massaging are used in the downstream process. A combination of bioprocesses for solvent recovery is required. Isolation of products such as ethanol, cysteic acid, and antibiotics is extracellular, but the isolation of enzymes within the cells requires cell destruction. has it. Cells are broken down by cell wall erosion. The destruction of the cell is used for the downstream recycling of the product.

Chromatography

Elimination of biomass from the extracted product is essential. Choosing a process, for example, straightening, discontinuous or continuous, vacuuming, crossover flow … Based on the nature of the product. The biodegradable mass is removed from the liquid, disposed of, or sold as a byproduct. For example, recombinant protein products that remain inside cells must be degraded and broken so that intracellular products are released. Four well-known methods for cell destruction include mechanical abrasive rubbing, turbulence, and high-speed stirring, high-pressure pumps and ultrasound waves, and non-mechanical techniques such as osmotic shock, freezing, and melting, cell wall enzyme digestion, and operations by Solvents and detergents. The very technique used to destroy a cell is to uniformize high pressure. The tension forces produced in this operation are sufficient to completely destroy a large variety of cells. The usual type is Moonton-Galin monolith. In this system, a high-pressure pump connects a confined valve to a closed and enclosed hole, through which the cells are driven at a pressure above 550 atmospheres. Uniformity is generally practical for cell destruction. The tap is closed if it is used for very stringent organisms. Stages involved and related to product recycling include:

  • Cell erosion for intracellular product recycling
  • Extracting or depleted cells
  • Remove soluble and unpolished substrate
  • Remove biomass from extracted product
  • Choose the type of straightener (discontinuous, continuous under vacuum, crossover, etc.)
  • Centrifugal (vertical, horizontal)
  • Sedimentation (and / or massification)

Medical Biochemistry Scope & Importance

The process depends on the fermentation solution conditions (temperature, pH, ionic strength), environmental components and the final state of the desired product

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