Biotechnology Fifth edition John E. Smith

Biotechnology Fifth edition John E. Smith Biotechnology is the major technology of the twenty-first century realize how much it impacts many aspects of human society,

Biotechnology Fifth edition John E. Smith

Biotechnology
Fifth edition

Biotechnology Fifth edition John E. Smith Biotechnology is the major technology of the twenty-first century – yet few people realize how much it impacts many aspects of human society. The defining aim of this new fifth edition is to re-establish the correct

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understanding of the term biotechnology. Biotechnology Fifth edition John E. Smith  Using the straightforward style that made the previous editions of his textbook so popular, John Smith once again helps students and general readers alike with the deciphering and use of biological knowledge. He explains the historical developments in biotechnology and the range of activities from brewing beer, the treatment of sewage and other wastes, and the creation of biofuels. He also discusses the innovations in molecular biology, genomics and proteomics, systems biology and their impact on new biotechnology. In this edition, John Smith also re-examines the ethics and morality of aspects of biotechnology and puts new emphasis on stem cells and regenerative medicine and micro RNA.

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Contents
Chapter 1 The nature of biotechnology 1
1.1 Introduction 1

GENETIC ENGINEERING AND BIOTECHNOLOGY

1.2 What is biotechnology? 2
1.3 Biotechnology: an interdisciplinary pursuit 6
1.4 Biotechnology: a three-component central core 13
1.5 Product safety 15
1.6 Public perception of biotechnology 17
1.7 Biotechnology and the developing world 17
Chapter 2 Biomass: a biotechnology substrate? 19
2.1 A biomass strategy 19
2.2 Natural raw materials 21
2.3 Availability of by-products 23
2.4 Raw materials and the future of biotechnology 25
Chapter 3 Genetics and biotechnology 29
3.1 Introduction 29
3.2 Industrial genetics 31

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3.3 Protoplast and cell fusion technologies 32
3.4 Genetic engineering 35
3.5 The polymerase chain reaction and DNA sequencing 41
3.6 Nucleic acid probes 43
3.7 Genomics and proteomics 44
3.8 Antisense and RNA interference 45
3.9 Systems biology 47
3.10 Potential laboratory biohazards of genetic engineering 47
Chapter 4 Bioprocess/fermentation technology 49
4.1 Introduction 49
4.2 Principles of microbial growth 53
4.3 The bioreactor 57
4.4 Scale-up 63
4.5 Media design for fermentation processes 63
4.6 Solid substrate fermentation 65
4.7 Technology of mammalian and plant cell culture 67
4.8 Metabolic engineering 70
4.9 Downstream processing 71

Biotechnology Fifth edition John E. Smith