Quantitative Analysis of DNA
Quantitative Analysis of DNA Aim: To determine the amount, concentration and purity of the given DNA sample Principle: This experiment is purely an application of the Beer Lamberts’ Law which states that the concentration of the sample is directly proportional to the absorbance of light done by the sample. It is given by the following expression A=E*C*l The device UV spectrophotometer works on this principle and used to find the concentration of the sample. Concentration and quality of a sample of DNA are measured with a UV spectrophotometer. A standard graph can be drawn using different concentrations of DNA and OD (optical density) values. The diagram above shows that a beam of monochromatic radiation (Io) is directed to a sample solution. Absorption takes place by the sample and the beam of radiation is leaving out (I) Materials Required: DNA sample TE buffer UV spectrophotometer PROCEDURE: Take …
