ABO BLOOD GROUPING Pdf
AIM:
To identify the monoclonal antibodies for phenotyping of human red blood cells.
PRINCIPLE:
To study haemagglutination of human blood grouping mouse monoclonal antibody raised against blood group A and B obtained by immunizing a mouse with red cell of blood group A and B and fusing the splenocytes of the mouse with myeloma cells Red blood cell antigen A or B when mixed with their respective antibodies leads agglutination determines the group of tested blood.
Fig: = ABO BLOOD GROUPING
CHARACTERISTICS OF ABO ANTIGENS:
ABO antigens are glycolipid in nature, meaning they are oligosaccharides attached directly to lipids on the red cell membrane.
These antigens stick out from red cell membrane and there are many antigens sites per red blood cell (approximately 800,000)
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