The One Thing
The One Thing Book Pdf “Be like a postage stamp-stick to one thing until you get there.” —Josh Billings The One Thing Book Pdf Click Here Full Book Pdf Download On June 7, 1991, the earth moved for 112 […]
The One Thing Book Pdf “Be like a postage stamp-stick to one thing until you get there.” —Josh Billings The One Thing Book Pdf Click Here Full Book Pdf Download On June 7, 1991, the earth moved for 112 […]
THE SEVEN HABITS OF HIGHLY EFFECTIVE PEOPLE Stephen Covey has written a remarkable book about the human condition, so elegantly written, so understanding of our embedded concerns, so useful for our organization and personal lives, that it’s going to
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LAW OF SUCCESS Full Book Pdf Download Free LAW OF SUCCESS Books to Read The 21st-Century Edition Revised and Updated NAPOLEON HILL Edited by Ann Hartley Bill Hartley The One Thing TRIBUTES TO “LAW OF SUCCESS” Full Book Pdf
Effect of UV Radiation on Bacterial Growth Aim: To Study the effect of UV exposure on the growth of bacterial cells. Principle: Mutations are a heritable change in the base sequence of DNA. Such mutations can be neutral or
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Quantitative Analysis of RNA Aim: To determine the amount and concentration of RNA sample isolated from bacterial cells Ligation of DNA fragments Principle: This experiment is purely an application of the Beer Lamberts’ Law which states that the concentration of the sample is directly proportional to the absorbance of light done by the sample. It is given by the following expression A = E*C*l The device UV spectrophotometer works on this principle and used to find the concentration of the sample Materials Required : RNA sample TE buffer UV spectrophotometer PROCEDURE: Remove a 10 µl aliquot of total RNA and dilute with 990 µl of TE buffer (10 mM Tris, 1 mM EDTA, pH 7.0). Restriction Enzyme Digestion Read at A260 and A280 blanked against TE buffer and calculate the amount of RNA obtained. The RNA obtained may be determined by the formula Total RNA (ug) = (A 260) (40 ug) (100) (0.05 ml) A260 is the absorbance of the solution at 260 nm
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Total RNA isolation from Bacterial Cells AIM: To isolate total RNA from the given bacterial culture PRINCIPLE: Total RNA is isolated and separated from DNA and protein after extraction with a solution called as Trizol (Sigma). Trizol is an
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Ligation of DNA fragments Aim: To perform the ligation of linearized T-vector with DNA fragment or the ligation of any restriction enzyme digested DNA fragments using T4 DNA ligase Principle: The basic strategy in molecular cloning is to insert
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Restriction Enzyme Digestion Aim: To digest the pUC18 DNA with BamH1 enzyme Principle: Restriction endonucleases are the class of enzymes that are used to cleave DNA at specific sites called Restriction sites. Every restriction enzyme has a specific restriction site at which it cuts a DNA molecule. For example restriction sequence for BamHI is GGATCC (type II restriction enzyme. The most abundantly used restriction enzymes are type II restriction enzymes which cleave at specific restriction site only. These endonucleases function adequately at pH 7.4 but different enzymes vary in their requirements for ionic strength usually provided by sodium chloride and magnesium chloride. It is also advisable to add a reducing agent such as dithiothreitol (DTT) which stabilizes the enzymes and
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Plasmid DNA Isolation Aim: To isolate plasmid DNA from bacterial cells Principle: When bacteria are lysed under alkaline conditions both DNA and proteins are precipitated. After the addition of acetate-containing neutralization buffer, the large and less supercoiled chromosomal DNA
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Qualitative Analysis of DNA separate and visualize DNA bands by Agarose gel electrophoresis Aim: To separate and visualize DNA bands by Agarose gel electrophoresis Introduction: Agarose gel electrophoresis is a powerful and widely used method that separates molecules on
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